Trypan blue Ophthalmic solution is used for intraocular use as a surgical aid during cataract and Vitreoretinal eye surgery. It is a blue dye that works by staining a part of the eye which creates a contrast between the different parts of the eye and helps the surgeon to see the different parts more clearly. It is a sterile, isotonic, solution and prepared by dissolving Trypan Blue powder in aqueous, isotonic solution. The pH range of the solution is maintained at 6.8 – 7.6.s